Introducing a new reporter gene, membrane-anchored Cypridina luciferase, for multiplex bioluminescence imaging.

ABSTRACT: Bioluminescence reporter gene imaging is a robust, high-throughput imaging modality that is useful for tracking cells and monitoring biological processes, both in cell culture and in small animals. We introduced and characterized a novel bioluminescence reporter-membrane-anchored Cypridina luciferase (maCLuc)-paired with a unique vargulin substrate. This luciferase-substrate pair has no cross-reactivity with established d-luciferin- or coelenterazine-based luciferase reporters. We compare maCLuc with several established luciferase-based reporter systems (firefly, click beetle, Renilla, and Gaussia luciferases), using both in vitro and in vivo models. We demonstrate the different imaging characteristics of these reporter systems, which allow for multiplexe

SUBMITTER: Moroz MA 

PROVIDER: S-EPMC8020342 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature