Temperature-Responsive Peptide-Nucleotide Coacervates.

ABSTRACT: Coacervates are a type of liquid-liquid phase separated (LLPS) droplets that can serve as models of membraneless organelles (MLOs) in living cells. Peptide-nucleotide coacervates have been widely used to mimic properties of ribonucleoprotein (RNP) granules, but the thermal stability and the role of base stacking is still poorly understood. Here, we report a systematic investigation of coacervates formed by five different nucleoside triphosphates (NTPs) with poly-l-lysine and poly-l-arginine as a function of temperature. All studied combinations exhibit an upper critical solution temperature (UCST), and a temperature-dependent critical salt concentration, originating from a significant nonelectrostatic contribution to the mixing free energy. Both the enthalpic and entropic parts of this non

SUBMITTER: Lu T 

PROVIDER: S-EPMC8020381 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature