Effect of cell microenvironment on the drug sensitivity of hepatocellular cancer cells.

ABSTRACT: The native hepatocellular cancer (HCC) microenvironment is characterized by more hypoxic, hypoglycemic, and acidic conditions than those used in standard cell culture. This study aimed to investigate whether HCC cells cultured in more native conditions have an altered phenotype and drug sensitivity compared to those cultured in standard conditions. Six HCC cell lines were cultured in "standard" (21% O₂, 25 mM glucose) or more "native" (1% O₂, 5 mM glucose, 10 mM lactate) conditions. Cells were assessed for growth rates, cell cycle distribution, relevant metabolite and protein levels, genome-wide gene expression, mitochondrial DNA sequence and sensitivity to relevant drugs. Many differences in cellular and molecular phenotypes and drug sensitivity were observed between

SUBMITTER: Bhattacharya B 

PROVIDER: S-EPMC8021027 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature