Differences in cytochrome p450 enzyme expression and activity in fetal and adult tissues.

ABSTRACT:

Introduction

Human cytochrome p450 (CYP) enzyme expression and activity is lower in the fetus as compared to the adult; however, limited quantitative data exists regarding the specific differences in magnitude or the degree of inducibility due to environmental factors.

Methods

We utilized a combination of in silico- and molecular-based approaches to profile and compare CYP expression/activity in human adult liver and fetal tissues. Using public datasets, we evaluated human CYP expression between: 1) placenta vs. adult livers; 2) fetal vs. adult livers; or 3) five compartments of the human placenta. We generated new experimental data, characterizing expression levels of nine CYPs in placenta/fetal liver vs. adult liver. In a subset of samples, we evaluated CYP3A4 activity. Fi

SUBMITTER: Robinson JF 

PROVIDER: S-EPMC8021394 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature