Decellularized Disc Hydrogels for hBMSCs tissue-specific differentiation and tissue regeneration.

ABSTRACT: Tissue specificity, a key factor in the decellularized tissue matrix (DTM), has shown bioactive functionalities in tuning cell fate-e.g., the differentiation of mesenchymal stem cells. Notably, cell fate is also determined by the living microenvironment, including material composition and spatial characteristics. Herein, two neighboring tissues within intervertebral discs, the nucleus pulposus (NP) and annulus fibrosus (AF), were carefully processed into DTM hydrogels (abbreviated DNP-G and DAF-G, respectively) to determine the tissue-specific effects on stem cell fate, such as specific components and different culturing methods, as well as in vivo regeneration. Distinct differences in their protein compositions were identified by proteomic analysis. Interestingly, the fate of human bone m

SUBMITTER: Peng Y 

PROVIDER: S-EPMC8022111 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature