Inflect: Optimizing Computational Workflows for Thermal Proteome Profiling Data Analysis.

ABSTRACT: The CETSA and Thermal Proteome Profiling (TPP) analytical methods are invaluable for the study of protein-ligand interactions and protein stability in a cellular context. These tools have increasingly been leveraged in work ranging from understanding signaling paradigms to drug discovery. Consequently, there is an important need to optimize the data analysis pipeline that is used to calculate protein melt temperatures (T_m) and relative melt shifts from proteomics abundance data. Here, we report a user-friendly analysis of the melt shift calculation workflow where we describe the impact of each individual calculation step on the final output list of stabilized and destabilized proteins. This report also includes a description of how key steps in the analysis workflow quant

SUBMITTER: McCracken NA 

PROVIDER: S-EPMC8022325 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature