Fast and inexpensive whole-genome sequencing library preparation from intact yeast cells.

ABSTRACT: Through the increase in the capacity of sequencing machines massively parallel sequencing of thousands of samples in a single run is now possible. With the improved throughput and resulting drop in the price of sequencing, the cost and time for preparation of sequencing libraries have become the major bottleneck in large-scale experiments. Methods using a hyperactive variant of the Tn5 transposase efficiently generate libraries starting from cDNA or genomic DNA in a few hours and are highly scalable. For genome sequencing, however, the time and effort spent on genomic DNA isolation limit the practicability of sequencing large numbers of samples. Here, we describe a highly scalable method for preparing high-quality whole-genome sequencing libraries directly from Saccharomyces cerevisiae cul

SUBMITTER: Vonesch SC 

PROVIDER: S-EPMC8022960 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature