Oligomerization of Sticholysins from Forster Resonance Energy Transfer.

ABSTRACT: Sticholysins are pore-forming toxins produced by sea anemones that are members of the actinoporin family. They exert their activity by forming pores on membranes, provided they have sphingomyelin. To assemble into pores, specific recognition, binding, and oligomerization are required. While recognition and binding have been extensively studied, delving into the oligomerization process and the stoichiometry of the pores has been more difficult. Here, we present evidence that these toxins are capable of oligomerizing in solution and suggesting that the interaction of sticholysin II (StnII) with its isoform sticholysin I (StnI) is stronger than that of StnI with itself. We also show that the stoichiometry of the final, thermodynamically stable StnI pores is, at least, heptameric. Furthermore,

SUBMITTER: Palacios-Ortega J 

PROVIDER: S-EPMC8023573 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature