Transcriptional Responses of Beauveria bassiana Blastospores Cultured Under Varying Glucose Concentrations.

ABSTRACT: Culturing the entomopathogenic fungus, Beauveria bassiana, under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphal growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remain elusive. A systematic transcriptome analysis of the differential gene expression was preformed to uncover the fungal transcriptomic response to osmotic and oxidative stresses associated with the resulting high blastospore yield. Differential gene expression was compared under moderate (10% w/v) and high (20% w/v) glucose concentrations daily for three days. The RNAseq-based transcriptomic results depicted a higher proportion of downregulated genes when the fungus was grown under 20% glucose than 10%.

SUBMITTER: Mascarin GM 

PROVIDER: S-EPMC8024584 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature