Kinetic Effects of β,γ-Modified Deoxynucleoside 5'-Triphosphate Analogues on RNA-Catalyzed Polymerization of DNA.

ABSTRACT: A recently described DNA polymerase ribozyme, obtained by in vitro evolution, provides the opportunity to investigate mechanistic features of RNA catalysis using methods that previously had only been applied to DNA polymerase proteins. Insight can be gained into the transition state of the DNA polymerization reaction by studying the behavior of various β,γ-bridging substituted methylene (CXY; X, Y = H, halo, methyl) or imido (NH) dNTP analogues that differ with regard to the pK_a4 of the bisphosphonate or imidodiphosphate leaving group. The apparent rate constant (k_pol) of the polymerase ribozyme was determined for analogues of dGTP and dCTP that span a broad range of acidities for the leaving group, ranging from 7.8 for the CF₂-bisphosp

SUBMITTER: Setterholm NA 

PROVIDER: S-EPMC8024617 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature