Frequent Transposition of Multiple Insertion Sequences in Geobacillus kaustophilus HTA426.

ABSTRACT: Geobacillus kaustophilus HTA426 is a thermophilic bacterium whose genome harbors numerous insertion sequences (IS). This study was initially conducted to generate mutant genes for thermostable T7 RNA polymerase in G. kaustophilus; however, relevant experiments unexpectedly identified that the organism transposed multiple IS elements and produced derivative cells that expressed a silent gene via transposition. The transposed elements were diverse and included members of the IS4, IS701, IS1634, and ISLre2 families. The transposition was relatively active at elevated temperatures and generated 4-9 bp of direct repeats at insertion sites. Transposition was more frequent in proliferative cells than in stationary cells but was comparable between both cel

SUBMITTER: Suzuki H 

PROVIDER: S-EPMC8024623 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature