Single-cell transcriptomics reveal the intratumoral landscape of infiltrated T-cell subpopulations in oral squamous cell carcinoma.

ABSTRACT: Systematic analysis of tumor-infiltrating lymphocytes is essential for the development of new cancer treatments and the prediction of clinical responses to immunotherapy. Immunomodulatory drugs are used for the treatment of oral squamous cell carcinoma (OSCC), depending on immune infiltration profiles of the tumor microenvironment. In this study, we isolated 11,866 single T cells from tumors and paired adjacent normal tissues of three patients with OSCC. Using single-cell RNA sequencing, we identified 14 distinct T-cell subpopulations within the tumors and 5 T-cell subpopulations in the adjacent normal tissues and delineated their developmental trajectories. Exhausted CD8⁺ T cells and regulatory CD4⁺ T cells (CD4⁺ Tregs) were enriched in OSCC tumors, potent

SUBMITTER: Chen J 

PROVIDER: S-EPMC8024729 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature