Targeting the YAP/TAZ Pathway in Uveal and Conjunctival Melanoma With Verteporfin.

ABSTRACT:

Purpose

The purpose of this study was to determine whether YAP/TAZ activation in uveal melanoma (UM) and the susceptibility of melanoma cell lines to YAP/TAZ inhibition by verteporfin (VP) is related to the tumor's genetic background.

Methods

Characteristics of 144 patients with enucleated UM were analyzed together with mRNA expression levels of YAP/TAZ-related genes (80 patients from the The Cancer Genome Atlas [TCGA] project and 64 patients from Leiden, The Netherlands). VP was administered to cell lines 92.1, OMM1, Mel270, XMP46, and MM28 (UM), CRMM1 and CRMM2 (conjunctival melanoma), and OCM3 (cutaneous melanoma). Viability, growth speed, and expression of YAP1-related proteins were assessed.

Results

In TCGA data, high expression of YAP1 and WWTR1 correlated with

SUBMITTER: Brouwer NJ 

PROVIDER: S-EPMC8024781 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature