Molecular characterization of advanced non-small cell lung cancer patients by cfDNA analysis: experience from routine laboratory practice.

ABSTRACT:

Background

Analysis of circulating free DNA (cfDNA) by the real-time PCR cobas^® EGFR Mutation Test v2 (cobas^® EGFR Test) is a diagnostic approach used in clinical practice for the characterization of advanced non-small cell lung cancer (NSCLC) patients. The test additionally outputs a semiquantitative index (SQI) which reflects the proportion of mutated versus wild-type copies of the EGFR gene in cfDNA with potential use as a biomarker. CfDNA concentration and cfDNA fragmentation pattern have also shown potential utility as biomarkers for cancer patients. We evaluated the implementation of EGFR testing and cfDNA related parameters in NSCLC patients in routine clinical setting as biomarkers for disease stage and diagnosis.

Methods

A prospec

SUBMITTER: Gonzalez de Aledo-Castillo JM 

PROVIDER: S-EPMC8024825 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature