Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model.

ABSTRACT:

Background

To describe the effect of mechanical ventilation on diaphragm mitochondrial oxygen consumption, ATP production, reactive oxygen species (ROS) generation, and cytochrome c oxidase activity and content, and their relationship to diaphragm strength in an experimental model of sepsis.

Methods

A cecal ligation and puncture (CLP) protocol was performed in 12 rats while 12 controls underwent sham operation. Half of the rats in each group were paralyzed and mechanically ventilated. We performed blood gas analysis and lactic acid assays 6 h after surgery. Afterwards, we measured diaphragm strength and mitochondrial oxygen consumption, ATP and ROS generation, and cytochrome c oxidase activity. We also measured malondialdehyde (MDA) content as an index of lipid peroxidation,

SUBMITTER: Eyenga P 

PROVIDER: S-EPMC8025065 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature