PRIMA‑1^met induces autophagy in colorectal cancer cells through upregulation of the mTOR/AMPK‑ULK1‑Vps34 signaling cascade.

ABSTRACT: p53‑reactivation and induction of massive apoptosis‑1, APR‑017 methylated (PRIMA‑1^met; APR246) targets mutant p53 to restore its wild‑type structure and function. It was previously demonstrated that PRIMA‑1^met effectively inhibited the growth of colorectal cancer (CRC) cells in a p53‑independent manner, and distinctly induced apoptosis by upregulating Noxa in p53‑mutant cell lines. The present study including experiments of western blotting, acridine orange staining and transmission electron microscopy revealed that PRIMA‑1^met induced autophagy in CRC cells independently of p53 status. Importantly, PRIMA‑1^met not only promoted autophagic vesicle (AV) formation and AV‑lysosome fusion, but also increased lysosomal degradation. Furthermore, Cell Cou

SUBMITTER: Li XL 

PROVIDER: S-EPMC8025076 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature