The role of intra-articular neuronal CCR2 receptors in knee joint pain associated with experimental osteoarthritis in mice.

ABSTRACT:

Background

C-C chemokine receptor 2 (CCR2) signaling plays a key role in pain associated with experimental murine osteoarthritis (OA) after destabilization of the medial meniscus (DMM). Here, we aimed to assess if CCR2 expressed by intra-articular sensory neurons contributes to knee hyperalgesia in the early stages of the model.

Methods

DMM surgery was performed in the right knee of 10-week-old male wild-type (WT), Ccr2 null, or Ccr2^RFP C57BL/6 mice. Knee hyperalgesia was measured using a Pressure Application Measurement device. CCR2 receptor antagonist (CCR2RA) was injected systemically (i.p.) or intra-articularly (i.a.) at different times after DMM to test its ability to reverse knee hyperalgesia. In vivo Ca²⁺ imaging of the dorsal root ganglion (DRG)

SUBMITTER: Ishihara S 

PROVIDER: S-EPMC8025346 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature