Subconjunctival administration of low-dose murine allogeneic mesenchymal stromal cells promotes corneal allograft survival in mice.

ABSTRACT:

Background

Systemic administration of mesenchymal stromal cells (MSCs) has been efficacious in many inflammatory disease settings; however, little data are available on the potential immunomodulatory effects following local MSC administration in the context of corneal transplantation. The purpose of this study was to assess the potential of subconjunctival injection of MSCs to promote corneal allograft survival.

Methods

MSCs were isolated from female C57BL/6 (H-2^k) or Balb/c (H-2^d) mice and extensively characterized. An allogeneic mouse corneal transplant model was used with Balb/c mice as recipients of C57BL/6 grafts. A dose-finding study starting with 5 × 10⁵ MSCs injected subconjunctivally at day - 7 was tested first followed by a more cli

SUBMITTER: Treacy O 

PROVIDER: S-EPMC8025388 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature