Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca²⁺ Signaling Pathway.

ABSTRACT: XueShuanTong (XST) comprising therapeutically active ginsenosides, a lyophilized extract of Panax notoginseng roots, is extensively used in traditional Chinese medicine to treat ischemic heart and cerebrovascular diseases. Our recent study shows that treatment with XST inhibits shear-induced thrombosis formation but the underlying mechanism remained unclear. This study aimed to investigate the hypothesis that XST inhibited shear-induced platelet aggregation via targeting the mechanosensitive Ca²⁺-permeable Piezo1 channel by performing platelet aggregation assay, Ca²⁺ imaging and Western blotting analysis. Exposure to shear at physiologically (1,000-2000 s^-1) and pathologically related rates (4,000-6,000 s^-1) induced platelet aggregation th

SUBMITTER: Liu L 

PROVIDER: S-EPMC8025832 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature