Versatile workflow for cell type-resolved transcriptional and epigenetic profiles from cryopreserved human lung.

ABSTRACT: Complexity of lung microenvironment and changes in cellular composition during disease make it exceptionally hard to understand molecular mechanisms driving development of chronic lung diseases. Although recent advances in cell type-resolved approaches hold great promise for studying complex diseases, their implementation relies on local access to fresh tissue, as traditional tissue storage methods do not allow viable cell isolation. To overcome these hurdles, we developed a versatile workflow that allows storage of lung tissue with high viability, permits thorough sample quality check before cell isolation, and befits sequencing-based profiling. We demonstrate that cryopreservation enables isolation of multiple cell types from both healthy and diseased lungs. Basal cells from cryopreserve

SUBMITTER: Llamazares-Prada M 

PROVIDER: S-EPMC8026197 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature