Read cloud sequencing elucidates microbiome dynamics in a hematopoietic cell transplant patient.

ABSTRACT: Low intestinal microbial diversity, often leading to domination of the intestine by a single organism, is associated with poor outcomes following hematopoietic cell transplantation (HCT). Understanding how certain organisms achieve domination in the intestine is limited by current metagenomic sequencing technologies, which are typically unable to reconstruct complete genome drafts without bacterial isolation and culture. Recently, we developed a metagenomic read cloud sequencing approach that provides significantly improved genome drafts for individual organisms compared to conventional short-read sequencing methods. Here, we apply read cloud sequencing to four longitudinal stool samples collected from an HCT patient before and after heavy antibiotic exposure. During this time period, the

SUBMITTER: Kang J 

PROVIDER: S-EPMC8026214 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature