Elevated EZH2 in ischemic heart disease epigenetically mediates suppression of Na_V1.5 expression.

ABSTRACT: Suppression of the cardiac sodium channel Na_V1.5 leads to fatal arrhythmias in ischemic heart disease (IHD). However, the transcriptional regulation of Na_V1.5 in cardiac ischemia is still unclear. Our studies are aimed to investigate the expression of enhancer of zeste homolog 2 (EZH2) in IHD and regulation of cardiac Na_V1.5 expression by EZH2. Human heart tissue was obtained from IHD and non-failing heart (NFH) patients; mouse heart tissue was obtained from the peri-infarct zone of hearts with myocardial infarction (MI) and hearts with a sham procedure. Protein and mRNA expression were measured by immunoblotting, immunostaining, and qRT-PCR. Protein-DNA binding and promoter activity were analyzed by ChIP-qPCR and luciferase assays, respectively. Na^+</

SUBMITTER: Zhao L 

PROVIDER: S-EPMC8026576 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature