Expression of novel acidic lipase from Micrococcus luteus in Pichia pastoris and its application in transesterification.

ABSTRACT:

Background

Lipases are promising biocatalysts for industrial applications and attract attention to be explored. A novel acidic lipase has been isolated from the lipolytic bacteria Micrococcus luteus EMP48-D (LipEMP48-D) screened from tempeh. The lipase gene had previously been overexpressed in Escherichia coli BL21, but the expression level obtained was relatively low. Here, to improve the expression level, the lipase gene was cloned to Pichia pastoris. We eliminated the native signal sequence of M. luteus and replaced it with α-mating factor (α-MF) signal sequence. We also optimized and synthesized the lipase gene based on codon preference in P. pastoris.

Results

LipEMP48-D lipase was expressed as an extracellular protein. Codon optimization has been conducted for 20 codons

SUBMITTER: Adina SR 

PROVIDER: S-EPMC8026790 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature