Comparative Analysis of Global Proteome and Lysine Acetylome Between Naive CD4⁺ T Cells and CD4⁺ T Follicular Helper Cells.

ABSTRACT: As a subgroup of CD4⁺ T helper cells, follicular helper T (Tfh) cells provide help to germinal center B cells and mediate the development of long-lived humoral immunity. Dysregulation of Tfh cells is associated with several major autoimmune diseases. Although recent studies showed that Tfh cell differentiation is controlled by the transcription factor Bcl6, cytokines, and cell-cell signals, limited information is available on the proteome and post-translational modifications (PTMs) of proteins in human Tfh cells. In the present study, we investigated quantitative proteome and acetylome in human naive CD4⁺ T cells and in vitro induced Tfh (iTfh) cells using the tandem mass tag (TMT) labeling technique, antibody-based affinity enrichment, and high-resolution liqu

SUBMITTER: Zhao M 

PROVIDER: S-EPMC8027069 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature