Comparison of Two Approaches for the Metataxonomic Analysis of the Human Milk Microbiome.

ABSTRACT: Recent work has demonstrated the existence of large inter-individual and inter-population variability in the microbiota of human milk from healthy women living across variable geographical and socio-cultural settings. However, no studies have evaluated the impact that variable sequencing approaches targeting different 16S rRNA variable regions may have on the human milk microbiota profiling results. This hampers our ability to make meaningful comparisons across studies. In this context, the main purpose of the present study was to re-process and re-sequence the microbiome in a large set of human milk samples (n = 412) collected from healthy women living at diverse international sites (Spain, Sweden, Peru, United States, Ethiopia, Gambia, Ghana and Kenya), by targeting a different 16S rRNA

SUBMITTER: Ruiz L 

PROVIDER: S-EPMC8027255 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature