A genome-wide copper-sensitized screen identifies novel regulators of mitochondrial cytochrome c oxidase activity.

ABSTRACT: Copper is essential for the activity and stability of cytochrome c oxidase (CcO), the terminal enzyme of the mitochondrial respiratory chain. Loss-of-function mutations in genes required for copper transport to CcO result in fatal human disorders. Despite the fundamental importance of copper in mitochondrial and organismal physiology, systematic identification of genes that regulate mitochondrial copper homeostasis is lacking. To discover these genes, we performed a genome-wide screen using a library of DNA-barcoded yeast deletion mutants grown in copper-supplemented media. Our screen recovered a number of genes known to be involved in cellular copper homeostasis as well as genes previously not linked to mitochondrial copper biology. These newly identified genes include the subunits of the

SUBMITTER: Garza NM 

PROVIDER: S-EPMC8027276 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature