Dataset Information

A strain of Phoma species improves drought tolerance of Pinus tabulaeformis.

ABSTRACT: Global warming has led to frequent droughts, posing challenges for afforestation in arid and semiarid regions. In search of labor-saving and money-saving methods to improve the survival and growth rates of trees in these regions, we isolated and identified fungal endophytes that can potentially enhance the drought-resistance abilities of seedlings. We isolated 93 endophytic strains from the roots of Pinus tabulaeformis trees grown on an arid cliff. Three isolates increased the drought resistance of the tree seedlings. Using morphological, molecular, and physiological-biochemical methods, we identified three isolates as different clones of a strain of Phoma spp. and studied the strain's effect on stress resistance-related substances in the seedlings. The results showed that the strain improved drought tolerance and increased the seedlings' proline levels and antioxidant enzyme activities. The strain also secreted abundant extracellular abscisic acid, which likely triggered the seedlings' protective mechanisms. This endophytic strain may provide a cheaper labor-saving, sustainable alternative to traditional methods of enhancing drought resistance.

SUBMITTER: Zhou XR

PROVIDER: S-EPMC8027514 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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A strain of Phoma species improves drought tolerance of Pinus tabulaeformis.

Zhou Xiu Ren XR Dai Lei L Xu Gui Fang GF Wang Hong Sheng HS

Scientific reports 20210407 1

Global warming has led to frequent droughts, posing challenges for afforestation in arid and semiarid regions. In search of labor-saving and money-saving methods to improve the survival and growth rates of trees in these regions, we isolated and identified fungal endophytes that can potentially enhance the drought-resistance abilities of seedlings. We isolated 93 endophytic strains from the roots of Pinus tabulaeformis trees grown on an arid cliff. Three isolates increased the drought resistance ...[more]

PMID: 33828138

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Project description:Understanding the factors affecting the growth of plantation forests can reduce the loss of economic and ecological values caused by plantation forest subhealth. Plantation forests are widely distributed in hilly areas with microtopographic features. Microtopography influences climatic factors associated with plant growth, during not only general time but also extreme events like droughts. However, little research has been conducted on the effects of microtopography on the plantation forest growth. In this paper, we selected Pinus tabulaeformis planted in a hilly site, and studied the effect of microtopography on the climate-growth relationship and drought response of a typical plantation in Northeast China using dendroecological methods. We found: 1) Between hill positions, temperature caused a climatic growth difference. Compared to the hilltop, the correlation of annual growth on the hillside with monthly temperature was more negative in July-August and less positive in January-April. 2) Between aspects, precipitation intensities caused a climatic growth difference. Compared to the sunny slope, the correlation of annual growth on the shady slope with monthly total precipitation below 10 mm/day was less positive (May-June) or more negative (March-April and July), while that with monthly total precipitation above 10 mm/day was more positive in most months.3) Drought response varied significantly based on hill position and aspect. There was no significant difference in resistance between hill positions, while recovery and resilience on the hilltop were greater than those on the hillside.Resistance, recovery, and resilience were all lower on the sunny slope than those on the shady slope. Overall, microtopography exists the effects on the growth of plantation forests, both in terms of climate-growth relationships in general climate and in response to drought when extreme events. Meanwhile, the climatic factors that caused the difference in growth of plantation forests between hill positions and aspects differed. The difference in growth between hill positions was caused by temperature, while that between aspects was caused by precipitation intensity. Drought response difference reflected the legacy effect of drought on plantation growth, which could lead to subsequent changes in climate-growth relationships. These findings demonstrate that strengthening the research of forest trees on microtopography is necessary for accurate carbon sink assessment and precise forest management.

Project description:Pinus taeda is an important forest tree species for plantations because of its rapid growth and high yield of oleoresins. Although P. taeda plantations distribute in warm and wet southern China, drought, sometime serious and long time, often occurs in the region. To explore drought tolerance of P. taeda and usage of beneficial microorganisms, P. taeda seedlings were planted in pots and were inoculated with root endophytic fungus Serendipita indica and finally were treated with drought stress for 53 d. Metabolome and proteome of their needles were analyzed. The results showed that S. indica inoculation of P. taeda seedlings under drought stress caused great changes in levels of some metabolites in their needles, especially some flavonoids and organic acids. Among them, the levels of eriocitrin, trans-aconitic acid, vitamin C, uric acid, alpha-ketoglutaric acid, vitamin A, stachydrine, coumalic acid, itaconic acid, calceolarioside B, 2-oxoglutaric acid, and citric acid were upregulated more than three times in inoculated seedlings under drought stress, compared to those of non-inoculated seedlings under drought stress. KEGG analysis showed that some pathways were enriched in inoculated seedlings under drought stress, such as flavonoid biosynthesis, ascorbate and aldarate metabolism, C5-branched dibasic acid metabolism. Proteome analysis revealed some specific differential proteins. Two proteins, namely, H9X056 and H9VDW5, only appeared in the needles of inoculated seedlings under drought stress. The protein H9VNE7 was upregulated more than 11.0 times as that of non-inoculated seedlings under drought stress. In addition, S. indica inoculation increased enrichment of water deficient-inducible proteins (such as LP3-1, LP3-2, LP3-3, and dehydrins) and those involved in ribosomal structures (such as A0A385JF23). Meanwhile, under drought stress, the inoculation caused great changes in biosynthesis and metabolism pathways, mainly including phenylpropanoid biosynthesis, cutin, suberine and wax biosynthesis, and 2-oxocarboxylic acid metabolism. In addition, there were positive relationships between accumulation of some metabolites and enrichment of proteins in P. taeda under drought stress. Altogether, our results showed great changes in metabolome and proteome in inoculated seedlings under drought stress and provided a guideline to further study functions of metabolites and proteins, especially those related to drought stress.

Dataset Information

A strain of Phoma species improves drought tolerance of Pinus tabulaeformis.

Publications

A strain of Phoma species improves drought tolerance of Pinus tabulaeformis.

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