Dual-Labeled Graphene Quantum Dot-Based Forster Resonance Energy Transfer Nanoprobes for Single-Molecule Localization Microscopy.

ABSTRACT: Single-molecule localization microscopy (SMLM)-based super-resolution imaging techniques (e.g., photoactivated localization microscopy (PALM)/stochastic optical reconstruction microscopy (STORM)) require that the employed optical nanoprobes possess fluorescence intensity fluctuations under certain excitation conditions. Here, we present a dual-labeled graphene quantum dot (GQD)-based Förster resonance energy transfer (FRET) nanoprobe, which is suitable for SMLM imaging. The nanoprobe is constructed by attaching Alexa Fluor 488 (AF488) and Alexa Fluor 568 (AF568) dye molecules onto GQDs. Experimental results confirmed the FRET effect of the nanoprobes. Moreover, under a single 405 nm excitation, the FRET nanoprobe exhibits excellent blinking behavior. SMLM imaging of microtubules in

SUBMITTER: Lu J 

PROVIDER: S-EPMC8028002 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature