Cyclodextrin/Adamantane-Mediated Targeting of Inoculated Bacteria in Mice.

ABSTRACT: Cyclodextrin (CD)-based host-guest interactions with adamantane (Ad) have demonstrated use for functionalizing living cells in vitro. The next step in this supramolecular functionalization approach is to explore the concept to deliver chemical cargo to living cells in vivo, e.g., inoculated bacteria, in order to study their dissemination. We validated this concept in two rodent Staphylococcus aureus models. Bacteria (1 × 10⁸ viable S. aureus) were inoculated by (1) intramuscular injection or (2) intrasplenic injection followed by dissemination throughout the liver. The bacteria were prefunctionalized with ^99mTc-UBI_29-41-Ad₂ (primary vector), which allowed us to both determine the bacterial load and create an in vivo

SUBMITTER: Welling MM 

PROVIDER: S-EPMC8028042 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature