CaBagE: A Cas9-based Background Elimination strategy for targeted, long-read DNA sequencing.

ABSTRACT: A substantial fraction of the human genome is difficult to interrogate with short-read DNA sequencing technologies due to paralogy, complex haplotype structures, or tandem repeats. Long-read sequencing technologies, such as Oxford Nanopore's MinION, enable direct measurement of complex loci without introducing many of the biases inherent to short-read methods, though they suffer from relatively lower throughput. This limitation has motivated recent efforts to develop amplification-free strategies to target and enrich loci of interest for subsequent sequencing with long reads. Here, we present CaBagE, a method for target enrichment that is efficient and useful for sequencing large, structurally complex targets. The CaBagE method leverages the stable binding of Cas9 to its DNA target to prot

SUBMITTER: Wallace AD 

PROVIDER: S-EPMC8031414 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature