Global biochemical analysis of plasma, serum and whole blood collected using various anticoagulant additives.

ABSTRACT:

Introduction

Analysis of blood for the evaluation of clinically relevant biomarkers requires precise collection and sample handling by phlebotomists and laboratory staff. An important consideration for the clinical application of metabolomics are the different anticoagulants utilized for sample collection. Most studies that have characterized differences in metabolite levels in various blood collection tubes have focused on single analytes. We define analyte levels on a global metabolomics platform following blood sampling using five different, but commonly used, clinical laboratory blood collection tubes (i.e., plasma anticoagulated with either EDTA, lithium heparin or sodium citrate, along with no additive (serum), and EDTA anticoagulated whole blood).

Methods

Using an unt

SUBMITTER: Kennedy AD 

PROVIDER: S-EPMC8031419 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature