Graph of graphs analysis for multiplexed data with application to imaging mass cytometry.

ABSTRACT: Imaging Mass Cytometry (IMC) combines laser ablation and mass spectrometry to quantitate metal-conjugated primary antibodies incubated in intact tumor tissue slides. This strategy allows spatially-resolved multiplexing of dozens of simultaneous protein targets with 1μm resolution. Each slide is a spatial assay consisting of high-dimensional multivariate observations (m-dimensional feature space) collected at different spatial positions and capturing data from a single biological sample or even representative spots from multiple samples when using tissue microarrays. Often, each of these spatial assays could be characterized by several regions of interest (ROIs). To extract meaningful information from the multi-dimensional observations recorded at different ROIs across different assays, we

SUBMITTER: Lin YE 

PROVIDER: S-EPMC8032202 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature