The neurosteroid allopregnanolone protects retinal neurons by effects on autophagy and GABRs/GABA_A receptors in rat glaucoma models.

ABSTRACT: In an ex vivo rat glaucoma model using dissected retinas, the neurosteroid allopregnanolone (AlloP) protects retinal ganglion cells (RGCs) via GABR/GABA_A receptors. To determine the involvement of macroautophagy/autophagy in neuroprotection by AlloP, we examined the effects of autophagy activators, rapamycin and torin 2, and autophagy inhibitors, bafilomycin A₁ and SAR405, on retinal retinal morphology and expression of MAP1 LC3B/LC3B (microtubule-associated protein 1 light chain 3 beta) and SQSTM1 (sequestosome 1). Administration of rapamycin or torin 2 exerted partial histological neuroprotection, while combined administration of AlloP with bafilomycin A₁ or SAR405 induced severe degeneration in a hyperbaric condition. Electron microscopic analyses

SUBMITTER: Ishikawa M 

PROVIDER: S-EPMC8032250 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature