Single Cell Label-Free Probing of Chromatin Dynamics During B Lymphocyte Maturation.

ABSTRACT: Large-scale intracellular signaling during developmental growth or in response to environmental alterations are largely orchestrated by chromatin within the cell nuclei. Chemical and conformational modifications of the chromatin architecture are critical steps in the regulation of differential gene expression and ultimately cell fate determination. Therefore, establishing chemical properties of the nucleus could provide key markers for phenotypic characterization of cellular processes on a scale of individual cells. Raman microscopy is a sensitive technique that is capable of probing single cell chemical composition-and sub-cellular regions-in a label-free optical manner. As such, it has great potential in both clinical and basic research. However, perceived limitations of Raman spectrosco

SUBMITTER: Morrish R 

PROVIDER: S-EPMC8033168 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature