Identification of SARS-CoV-2 inhibitors using lung and colonic organoids.

ABSTRACT: There is an urgent need to create novel models using human disease-relevant cells to study severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) biology and to facilitate drug screening. Here, as SARS-CoV-2 primarily infects the respiratory tract, we developed a lung organoid model using human pluripotent stem cells (hPSC-LOs). The hPSC-LOs (particularly alveolar type-II-like cells) are permissive to SARS-CoV-2 infection, and showed robust induction of chemokines following SARS-CoV-2 infection, similar to what is seen in patients with COVID-19. Nearly 25% of these patients also have gastrointestinal manifestations, which are associated with worse COVID-19 outcomes¹. We therefore also generated complementary hPSC-derived colonic organoids (hPSC-COs) to explore the response

SUBMITTER: Han Y 

PROVIDER: S-EPMC8034380 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature