Enhancing Comprehensive Analysis of Secreted Glycoproteins from Cultured Cells without Serum Starvation.

ABSTRACT: Glycoproteins secreted by cells play essential roles in the regulation of extracellular activities. Secreted glycoproteins are often reflective of cellular status, and thus glycoproteins from easily accessible bodily fluids can serve as excellent biomarkers for disease detection. Cultured cells have been extensively employed as models in the research fields of biology and biomedicine, and global analysis of glycoproteins secreted from these cells provides insights into cellular activities and glycoprotein functions. However, comprehensive identification and quantification of secreted glycoproteins is a daunting task because of their low abundances compared with the high-abundance serum proteins required for cell growth and proliferation. Several studies employed serum-free media to analyze

SUBMITTER: Suttapitugsakul S 

PROVIDER: S-EPMC8034805 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature