Automated 3D scoring of fluorescence in situ hybridization (FISH) using a confocal whole slide imaging scanner.

ABSTRACT: Fluorescence in situ hybridization (FISH) is a technique to visualize specific DNA/RNA sequences within the cell nuclei and provide the presence, location and structural integrity of genes on chromosomes. A confocal Whole Slide Imaging (WSI) scanner technology has superior depth resolution compared to wide-field fluorescence imaging. Confocal WSI has the ability to perform serial optical sections with specimen imaging, which is critical for 3D tissue reconstruction for volumetric spatial analysis. The standard clinical manual scoring for FISH is labor-intensive, time-consuming and subjective. Application of multi-gene FISH analysis alongside 3D imaging, significantly increase the level of complexity required for an accurate 3D analysis. Therefore, the purpose of this study is to establish

SUBMITTER: Frankenstein Z 

PROVIDER: S-EPMC8035347 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature