Molecular characterization of porcine group A rotavirus to contain piglet diarrhea for productivity enhancement in North East India.

ABSTRACT: Gastrointestinal tract infections with acute diarrhea are indeed a serious cause of concern that held back the swine industry of North East India and among the various etiological agents causing the disease, group A rotavirus is the most common. In this regard, molecular characterization of the viral strains circulating in the region is of utmost importance for a strategic approach to control the disease. Thus, in the present study, diarrheic fecal samples from piglets of Tripura and Assam were evaluated for genotypic characterization of VP7 and VP4 gene of the virus. The samples positive for group A rotavirus were subjected to G and P type determination by nested- multiplex RT PCR as well as nucleotide sequencing of VP7 and VP4 gene. Nested- multiplex RT PCR revealed the presence of porci

SUBMITTER: Das S 

PROVIDER: S-EPMC8035605 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature