Glycoconjugate vaccines against Salmonella enterica serovars and Shigella species: existing and emerging methods for their analysis.

ABSTRACT: The global spread of enteric disease, the increasingly limited options for antimicrobial treatment and the need for effective eradication programs have resulted in an increased demand for glycoconjugate enteric vaccines, made with carbohydrate-based membrane components of the pathogen, and their precise characterisation. A set of physico-chemical and immunological tests are employed for complete vaccine characterisation and to ensure their consistency, potency, safety and stability, following the relevant World Health Organization and Pharmacopoeia guidelines. Variable requirements for analytical methods are linked to conjugate structure, carrier protein nature and size and O-acetyl content of polysaccharide. We investigated a key stability-indicating method which measures the perce

SUBMITTER: Bazhenova A 

PROVIDER: S-EPMC8035613 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature