Accuracy of the serological detection of IgG and IgM to SARS-Cov-2: a prospective, cross-sectional study.

ABSTRACT: In response to the rapidly evolving of SARS-CoV-2 infection, numerous serological tests have been developed but their sensitivity and specificity are unclear. We collected serum samples of patients and health-care professionals to assess the accuracy of chemiluminescent (CLIA) and two lateral flow immunochromatographic assays (LFIA) to determine IgG and IgM antibodies to SARS-CoV-2 virus. We calculated the φ correlation for qualitative results and test accuracy, adopting the following case definition: either real-time-PCR positivity or serological positivity with at least two different tests. We analyzed 259 samples, obtaining strong correlation between CLIA and both LFIA for IgG (φ=0.9), and moderate correlation for IgM (φ=0.6). For patients, the sensitivity was suboptimal for all methods

SUBMITTER: Pecoraro V 

PROVIDER: S-EPMC8036011 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature