N-Terminal Acetyltransferase Naa40p Whereabouts Put into N-Terminal Proteoform Perspective.

ABSTRACT: The evolutionary conserved N-alpha acetyltransferase Naa40p is among the most selective N-terminal acetyltransferases (NATs) identified to date. Here we identified a conserved N-terminally truncated Naa40p proteoform named Naa40p25 or short Naa40p (Naa40^S). Intriguingly, although upon ectopic expression in yeast, both Naa40p proteoforms were capable of restoring N-terminal acetylation of the characterized yeast histone H2A Naa40p substrate, the Naa40p histone H4 substrate remained N-terminally free in human haploid cells specifically deleted for canonical Naa40p27 or 237 amino acid long Naa40p (Naa40^L), but expressing Naa40^S. Interestingly, human Naa40^L and Naa40^S displayed differential expression and subcellular localization patterns

SUBMITTER: Jonckheere V 

PROVIDER: S-EPMC8037211 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature