Ex vivo normothermic perfusion of isolated segmental porcine bowel: a novel functional model of the small intestine.

ABSTRACT:

Background

There is an unmet need for suitable ex vivo large animal models in experimental gastroenterology and intestinal transplantation. This study details a reliable and effective technique for ex vivo normothermic perfusion (EVNP) of segmental porcine small intestine.

Methods

Segments of small intestine, 1.5-3.0 m in length, were retrieved from terminally anaesthetized pigs. After a period of cold ischaemia, EVNP was performed for 2 h at 37°C with a mean pressure of 80 mmHg using oxygenated autologous blood diluted with Ringer's solution. The duration of EVNP was extended to 4 h for a second set of experiments in which two segments of proximal to mid-ileum (1.5-3.0 m) were retrieved from each animal and reperfused with whole blood (control) or leucocyte-depleted blood t

SUBMITTER: Hamed MO 

PROVIDER: S-EPMC8038264 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature