The Photosystem II Assembly Factor Ycf48 from the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803 Is Lipidated Using an Atypical Lipobox Sequence.

ABSTRACT: Photochemical energy conversion during oxygenic photosynthesis is performed by membrane-embedded chlorophyll-binding protein complexes. The biogenesis and maintenance of these complexes requires auxiliary protein factors that optimize the assembly process and protect nascent complexes from photodamage. In cyanobacteria, several lipoproteins contribute to the biogenesis and function of the photosystem II (PSII) complex. They include CyanoP, CyanoQ, and Psb27, which are all attached to the lumenal side of PSII complexes. Here, we show that the lumenal Ycf48 assembly factor found in the cyanobacterium Synechocystis sp. PCC 6803 is also a lipoprotein. Detailed mass spectrometric analysis of the isolated protein supported by site-directed mutagenesis experiments indicates lipidation of t

SUBMITTER: Knoppova J 

PROVIDER: S-EPMC8038367 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature