The human aortic endothelium undergoes dose-dependent DNA methylation in response to transient hyperglycemia.

ABSTRACT:

Background

Glycemic control is a strong predictor of long-term cardiovascular risk in patients with diabetes mellitus, and poor glycemic control influences long-term risk of cardiovascular disease even decades after optimal medical management. This phenomenon, termed glycemic memory, has been proposed to occur due to stable programs of cardiac and endothelial cell gene expression. This transcriptional remodeling has been shown to occur in the vascular endothelium through a yet undefined mechanism of cellular reprogramming.

Methods

In the current study, we quantified genome-wide DNA methylation of cultured human endothelial aortic cells (HAECs) via reduced-representation bisulfite sequencing (RRBS) following exposure to diabetic (250 mg/dL), pre-diabetic (125 mg/dL), or eugly

SUBMITTER: Pepin ME 

PROVIDER: S-EPMC8038422 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature