HMEJ-based safe-harbor genome editing enables efficient generation of cattle with increased resistance to tuberculosis.

ABSTRACT: The CRISPR/Cas9 system has been used in a wide range of applications in the production of gene-edited animals and plants. Most efforts to insert genes have relied on homology-directed repair (HDR)-mediated integration, but this strategy remains inefficient for the production of gene-edited livestock, especially monotocous species such as cattle. Although efforts have been made to improve HDR efficiency, other strategies have also been proposed to circumvent these challenges. Here we demonstrate that a homology-mediated end-joining (HMEJ)-based method can be used to create gene-edited cattle that displays precise integration of a functional gene at the ROSA26 locus. We found that the HMEJ-based method increased the knock-in efficiency of reporter genes by eightfold relative to the tradition

SUBMITTER: Yuan M 

PROVIDER: S-EPMC8038940 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature