Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast.

ABSTRACT: Saccharomyces cerevisiae plays an important role in the heterologous expression of an array of proteins due to its easy manipulation, low requirements and ability for protein post-translational modifications. The implementation of the preproleader secretion signal of the α-factor mating pheromone from this yeast contributes to increase the production yields by targeting the foreign protein to the extracellular environment. The use of this signal peptide combined with enzyme-directed evolution allowed us to achieve the otherwise difficult functional expression of fungal laccases in S. cerevisiae, obtaining different evolved α-factor preproleader sequences that enhance laccase secretion. However, the design of a universal signal peptide to enhance the production of heterologous proteins in S

SUBMITTER: Aza P 

PROVIDER: S-EPMC8038962 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature