Deletion of Alox15 improves kidney dysfunction and inhibits fibrosis by increased PGD₂ in the kidney.

ABSTRACT:

Background

Lipid-metabolizing enzymes and their metabolites affect inflammation and fibrosis, but their roles in chronic kidney disease (CKD) have not been completely understood.

Methods

To clarify their role in CKD, we measured the mRNA levels of major lipid-metabolizing enzymes in 5/6 nephrectomized (Nx) kidneys of C57BL/6 J mice. Mediator lipidomics was performed to reveal lipid profiles of CKD kidneys.

Results

In 5/6 Nx kidneys, both mRNA and protein levels of Alox15 were higher when compared with those in sham kidneys. With respect to in situ hybridization, the mRNA level of Alox15 was higher in renal tubules of 5/6 Nx kidneys. To examine the role of Alox15 in CKD pathogenesis, we performed 5/6 Nx on Alox15^-/- mice. Alox15^-/- CKD mice exhibi

SUBMITTER: Takahashi N 

PROVIDER: S-EPMC8038997 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature