Staphylococcus aureus β-Hemolysin Up-Regulates the Expression of IFN-γ by Human CD56^bright NK Cells.

ABSTRACT: IFN-γ is produced upon stimulation with S. aureus and may play a detrimental role during infection. However, whether hemolysins play a role in the mechanism of IFN-γ production has not been fully characterized. In this study, we demonstrated that Hlb, one of the major hemolysins of S. aureus, upregulated IFN-γ production by CD56^bright NK cells from human peripheral blood mononuclear cells (PBMCs). Further investigation showed that Hlb increased calcium influx and induced phosphorylation of ERK1/2. Either blocking calcium or specifically inhibiting phosphorylation of ERK1/2 decreased the production of IFN-γ induced by Hlb. Moreover, we found that this process was dependent on the sphingomyelinase activity of Hlb. Our findings revealed a novel mechanism of IFN-γ prod

SUBMITTER: Guan Z 

PROVIDER: S-EPMC8039520 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature