Proteasome activator Blm10 levels and autophagic degradation directly impact the proteasome landscape.

ABSTRACT: The proteasome selectively degrades proteins. It consists of a core particle (CP), which contains proteolytic active sites that can associate with different regulators to form various complexes. How these different complexes are regulated and affected by changing physiological conditions, however, remains poorly understood. In this study, we focused on the activator Blm10 and the regulatory particle (RP). In yeast, increased expression of Blm10 outcompeted RP for CP binding, which suggests that controlling the cellular levels of Blm10 can affect the relative amounts of RP-bound CP. While strong overexpression of BLM10 almost eliminated the presence of RP-CP complexes, the phenotypes this should induce were not observed. Our results show this was due to the induction of Blm10-CP autophagy u

SUBMITTER: Burris A 

PROVIDER: S-EPMC8039559 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature