Analysis of metabolic network disruption in engineered microbial hosts due to enzyme promiscuity.

ABSTRACT: Increasing understanding of metabolic and regulatory networks underlying microbial physiology has enabled creation of progressively more complex synthetic biological systems for biochemical, biomedical, agricultural, and environmental applications. However, despite best efforts, confounding phenotypes still emerge from unforeseen interplay between biological parts, and the design of robust and modular biological systems remains elusive. Such interactions are difficult to predict when designing synthetic systems and may manifest during experimental testing as inefficiencies that need to be overcome. Transforming organisms such as Escherichia coli into microbial factories is achieved via several engineering strategies, used individually or in combination, with the goal of maximizing t

SUBMITTER: Porokhin V 

PROVIDER: S-EPMC8039717 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature